The presence of pesticide residues in vegetables is growing concern for the producers, consumers. These pesticide residues can persist to the harvest stage, making possible the contamination of vegetables which may be harmful to human health due to consumption of contaminated vegetables. A rapid, sensitive, simple and reliable HPLC method was developed to determine Imidacloprid residue in extract of vegetable. All analyses were performed binary gradient HPLC with UV detection at 270 nm using a mobile phase of acetonitrile: water (20:80, v/v). Recoveries were found to be in the 98-103% range. The method consisted of extracting sample with methanol and development of method for determination of Imidacloprid by HPLC. Imidacloprid concentration in vegetables extract was detected by HPLC which was found to be 9.83μg/ml and 10.04μg/ml. Imidacloprid concentration in vegetables extract was detected by HPLC which was found in small concentration which is not expected to cause health effects on humans when merit is applied as instructed on the label of pesticide.
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